Molecular Biology Reagents

 


Loading dyes are essential for the preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels. Depending on your DNA fragment of interest you can chose between different dyes that prevent DNA masking during gel exposure to UV light.

DNA loading dyes normally contain:

  • Glycerol to ensure that the sample easily sinks into the well
  • EDTA, which binds divalent metal ions that may interfere with electrophoresis. By complexing metal ions, EDTA also stops metal-dependent enzymatic reactions such as DNA degradation by nucleases
  • Tracking dyes (bromophenol blue, xylene cyanol FF, or orange G) to monitor the progress of electrophoresis by the migration of the dyes

DNA ladders prepared with various loading dye


Product offerings

Loading dye Description Quantity Catalog #
6X DNA Loading Dye Two-color tracking of DNA migration during electrophoresis 5 x 1 mL R0611
6X MassRuler DNA Loading Dye One-color tracking of DNA migration during electrophoresis 5 x 1 mL R0621
6X Orange DNA Loading Dye Two-color tracking of DNA migration during electrophoresis 5 x 1 mL R0631
6X DNA Loading Dye & SDS Solution Two-color tracking of DNA migration during electrophoresis 5 x 1 mL R1151



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